General Cell Based Assays
Eurofins Discoverx PathHunter eXpress P2RY6 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0500E2CP19M)
The PathHunter P2RY6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. UTP) based activation of P2RY6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged P2RY6 and EA-tagged β-Arrestin-2. Activation of P2RY6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rOPRM1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0494E2RCP2L)
The PathHunter rOPRM1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. DADLE) based activation of rOPRM1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rOPRM1 and EA-tagged β-Arrestin-2. Activation of rOPRM1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter IGF1R Bioassay Kit, 2-Plate. (93-0505Y1-00069)
The PathHunter IGF1R Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This cell line enables assessment of ligand (e.g. IGF1, IGF2, agonist antibody) based activation of IGF1R receptors via detection of SH2 domain protein recruitment. The included cell line overexpresses ProLink-tagged IGF1R target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress mGLP1R CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0478E2MCP0L)
The PathHunter mGLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of mGLP1R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mGLP1R and EA-tagged β-Arrestin-2. Activation of mGLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Raybiotech Inc Human Sp1 Activity Assay Kit, 1 Plate Kit
RayBio® Human Sp1 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical Sp1 binding sequences to semi-quantitatively detect active Sp1 in lysates or nuclear extracts. Dry ice shipment (additional fee).
Abcam WST Substrate Mix (lyophilized).
WST Substrate Mix (lyophilized).
The product is subject to the following: Abcam Restricted Use Statement
STEMCELL Technologies ALDHbr Assay Kit, For labeling: 6 x 10^7 cells
The ALDHbr Assay Kit is optimized for the identification and quantitation of CD34+ cells that express high levels of the enzyme aldehyde dehydrogenase (ALDH) in human cord blood samples. This kit contains both the CD34+ Cell Detection Kit and the ALDEFLUOR™ Kit. The CD34+ Cell Detection Kit contains pre-selected fluorochrome-conjugated monoclonal antibodies that have been optimized for use with the ALDEFLUOR™ Kit to detect cells that express CD34 and have high ALDH activity by flow cytometry. The CD34+ Cell Detection Kit contains anti-human antibodies APC CD34, PE CD45, and PE-Cyanine5 Glycophorin A/B, and 7-AAD viability dye.A flow cytometer equipped with a 488 nm laser and a 635 nm laser for excitation and appropriate filters for detecting APC, PE, PE-Cyanine5 and 7-AAD fluorescence is required for use.
Eurofins Discoverx PathHunter eXpress mCXCR6 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0512E2MCP0M)
The PathHunter mCXCR6 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL16) based activation of mCXCR6 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR6 and EA-tagged β-Arrestin-2. Activation of mCXCR6 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Amsbio LLC Heparanase Assay Kit without Positive Control Enzyme
Measure enzyme activity of heparanase in cell culture supernatants, human plasma, biological fluids and tissue samples. These kits provide a solution to the performance and standardization problems found with traditional measurement techniques used for these types of enzymes.
Cayman Chemical MItoLIteOrnge FX570 500test
A mitochondrial dye for live and fixed cells; ex/em = 545/575 nm; has been used to label mitochondria in Hep2 cells treated with cisplatin and NAC
Eurofins Discoverx PathHunter eXpress IFNGR1/IFNGR2 Dimerization Assay, 2pl Kit. (93-1098E3CP5M)
The PathHunter IFNGR1/IFNGR2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IFN-g) induced dimerization of IFNGR1/IFNGR2 receptors. This product uses EFC technology. The cell line included overexpresses IFNGR1 target protein fused to PK alongside IFNGR2 target protein fused to EA. Upon ligand-induced dimerization of IFNGR1/IFNGR2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human Total cGAS Detection Kit, Control Lysate
HTRF Human Total cGAS Detection Kit, Control Lysate